A new generation of U.S.-based AI startups is making waves, founded after 2015 and now armed with $10M–$50M in funding. These companies are turning cutting-edge research into real-world products, tackling challenges across healthcare, finance, logistics, and more. Their fresh perspective and strong backing are accelerating AI’s next leap forward.
The New Faces of American AI Innovation
From smarter automation to ethical AI and advanced data intelligence, these startups are redefining the role of artificial intelligence in business and everyday life.
Let’s explore the rising stars reshaping the U.S. tech landscape.
| Company | Founded | Location | Employees | Last Funding | Last Amount | Total Funding |
|---|---|---|---|---|---|---|
| Trust Stamp | 2015 | Atlanta, Georgia | 101–250 | Dec 5, 2024 | $3M | $33.34M |
| Attentive | 2017 | Wilmington, Delaware | 251–500 | Feb 8, 2024 | $7M | $14M |
| Ethyca | 2018 | New York, New York | 11–50 | Dec 5, 2024 | $10M | $37.5M |
| Vivoo | 2017 | San Francisco, California | 51–100 | Jul 15, 2024 | $10M | $18.41M |
| Pepper | 2019 | New York, New York | 11–50 | May 13, 2024 | $30M | $46M |
| Stella Automotive AI | 2016 | Tampa, Florida | 11–50 | Dec 5, 2024 | $11M | $34.7M |
| Neurable | 2015 | Boston, Massachusetts | 11–50 | May 7, 2024 | $13M | $22.76M |
| Canary Speech | 2016 | Provo, Utah | 11–50 | Jun 12, 2024 | $13M | $18.27M |
| NLX | 2018 | New York, New York | 11–50 | Feb 26, 2024 | $12M | $21.6M |
| Amesite | 2017 | Detroit, Michigan | 11–50 | Jan 7, 2025 | $3.6M | $40.3M |
| Antithesis | 2018 | Vienna, Virginia | 11–50 | Feb 13, 2024 | $47M | $47M |
| Rain AI | 2017 | Redwood City, California | 11–50 | May 26, 2024 | $8.1M | $40.23M |
| Reflexivity | 2019 | New York, New York | 11–50 | Oct 29, 2024 | $30M | $42.75M |
| PictorLabs | 2019 | Los Angeles, California | 11–50 | Sep 18, 2024 | $30M | $48.815M |
| Segmed | 2019 | Palo Alto, California | 11–50 | Sep 3, 2024 | $10.4M | $20.42M |
| Bolster AI | 2019 | Santa Clara, California | 51–100 | May 22, 2024 | $14M | $46M |
| Epistemic AI | 2018 | Westport, Connecticut | 11–50 | Jan 13, 2025 | $4M | $11.39M |
| Numa | 2017 | Oakland, California | 11–50 | Oct 1, 2024 | $32M | $44.1M |
| Ezra AI | 2018 | New York, New York | 11–50 | Feb 7, 2024 | $21M | $43M |
| Reveal Technology | 2018 | San Francisco, California | 11–50 | Dec 11, 2024 | $11.2M | $11.95M |
1. Trust Stamp
- Founded: 2015
- Location: Atlanta, Georgia, USA
- Industry: Artificial Intelligence (AI), Biometrics
- Number of Employees: 101–250
- Last Funding Amount: $3M (Latest Round: December 5, 2024)
- Total Funding Amount: $33.34M
Trust Stamp is a privacy-first AI and biometric technology company focused on providing secure identity authentication solutions. Leveraging proprietary AI algorithms and advanced biometrics, the platform enables organizations to verify identities without compromising personal data. Used across sectors like banking, travel, and law enforcement, Trust Stamp’s solutions enhance fraud prevention, compliance, and customer onboarding while maintaining high standards of data privacy and ethical AI use.
2. Attentive
- Founded: May 18, 2017
- Location: Wilmington, Delaware, USA
- Industry: Artificial Intelligence (AI), Information Technology
- Number of Employees: 251–500
- Last Funding Amount: $7M (Latest Round: February 8, 2024)
- Total Funding Amount: $14M
Attentive is an AI-powered technology company focused on automating enterprise communications and enhancing customer engagement. Its platform integrates natural language processing and machine learning to deliver personalized, real-time messaging solutions for businesses. By streamlining interactions across digital channels, Attentive helps organizations improve response times, optimize workflows, and increase customer satisfaction while reducing operational costs.
3. Ethyca
- Founded: 2018
- Location: New York, New York, USA
- Industry: Artificial Intelligence (AI), Compliance
- Number of Employees: 11–50
- Last Funding Amount: $10M (Latest Round: December 5, 2024)
- Total Funding Amount: $37.5M
Ethyca is a privacy infrastructure company leveraging AI to help businesses automate data compliance and governance. Its platform provides tools for consent management, data mapping, and subject rights fulfillment, ensuring adherence to global privacy laws like GDPR and CCPA. By integrating seamlessly into existing systems, Ethyca empowers organizations to responsibly manage user data, reduce legal risk, and build customer trust through transparent data practices.
4. Vivoo
- Founded: June 2017
- Location: San Francisco, California, USA
- Industry: Artificial Intelligence (AI), Health Care
- Number of Employees: 51–100
- Last Funding Amount: $10M (Latest Round: July 15, 2024)
- Total Funding Amount: $18.41M
Vivoo develops personalized wellness solutions using AI-powered urine test strips and a mobile app to provide real-time health insights. The platform analyzes biomarkers related to hydration, vitamins, and nutrition, offering customized dietary and lifestyle recommendations. Vivoo empowers users to track and improve their well-being conveniently from home, promoting proactive health management.
5. Pepper
- Founded: 2019
- Location: New York, New York, USA
- Industry: Artificial Intelligence (AI), E-Commerce
- Number of Employees: 11–50
- Last Funding Amount: $30M (Latest Round: May 13, 2024)
- Total Funding Amount: $46M
Pepper is a B2B e-commerce platform leveraging AI to streamline the food supply chain, enabling distributors and restaurants to manage orders, inventory, and payments more efficiently. By automating workflows and providing real-time analytics, Pepper enhances operational efficiency and customer experience for the food service industry.
6. Stella Automotive AI
- Founded: 2016
- Location: Tampa, Florida, USA
- Industry: Artificial Intelligence (AI), Automotive
- Number of Employees: 11–50
- Last Funding Amount: $11M (Latest Round: December 5, 2024)
- Total Funding Amount: $34.7M
Stella Automotive AI specializes in AI-driven solutions for the automotive industry, offering advanced technologies for autonomous driving, vehicle safety, and predictive maintenance. Their platform uses machine learning algorithms to optimize vehicle performance and provide real-time analytics to improve operational efficiency. With a focus on enhancing the driving experience and safety, Stella Automotive AI is helping shape the future of smart mobility and autonomous vehicles.
7. Neurable
- Founded: 2015
- Location: Boston, Massachusetts, USA
- Industry: Artificial Intelligence (AI), Augmented Reality
- Number of Employees: 11–50
- Last Funding Amount: $13M (Latest Round: May 7, 2024)
- Total Funding Amount: $22.76M
Neurable is at the forefront of brain-computer interface (BCI) technology, creating innovative solutions that merge artificial intelligence with augmented reality. The company focuses on developing mind-controlled interfaces that allow users to interact with virtual environments and devices through thought alone. Neurable’s AI-powered systems aim to revolutionize industries such as gaming, health care, and neurotechnology by enhancing user experience and accessibility through seamless brainwave interaction.
8. Canary Speech
- Founded: 2016
- Location: Provo, Utah, USA
- Industry: Artificial Intelligence (AI), Health Care
- Number of Employees: 11–50
- Last Funding Amount: $13M (Latest Round: June 12, 2024)
- Total Funding Amount: $18.27M
Canary Speech leverages AI and machine learning to develop speech recognition technology that aids in early diagnosis and monitoring of neurological and mental health conditions. Their platform analyzes speech patterns to detect signs of diseases like Alzheimer’s, Parkinson’s, and depression. By integrating advanced AI models, Canary Speech helps health care professionals monitor patients’ conditions and predict cognitive decline, enhancing patient care through innovative speech-based diagnostics.
9. NLX
- Founded: March 2018
- Location: New York, New York, USA
- Industry: Artificial Intelligence (AI), Customer Service
- Number of Employees: 11–50
- Last Funding Amount: $12M (Latest Round: February 26, 2024)
- Total Funding Amount: $21.6M
NLX specializes in AI-driven customer service solutions, offering voice and chat-based platforms that enhance customer interaction through natural language processing. Their intelligent automation tools are designed to streamline customer support, improve engagement, and reduce operational costs. By using AI to understand and respond to customer inquiries in real time, NLX transforms the way businesses provide service and support, ensuring a more efficient and personalized experience for customers across various industries.
10. Amesite
- Founded: 2017
- Location: Detroit, Michigan, USA
- Industry: Artificial Intelligence (AI), Computer
- Number of Employees: 11–50
- Last Funding Amount: $3.6M (Latest Round: January 7, 2025)
- Total Funding Amount: $40.3M
Amesite leverages AI to build scalable and customizable learning management systems (LMS) for enterprises, higher education institutions, and other organizations. Their platform enhances online learning experiences by offering tailored, AI-driven solutions that improve engagement, track progress, and streamline educational processes. Amesite’s innovative use of AI technology allows for continuous optimization of learning programs, helping clients better manage their workforce development and educational goals.
11. Antithesis
- Founded: 2018
- Location: Vienna, Virginia, USA
- Industry: Artificial Intelligence (AI), Information Technology
- Number of Employees: 11–50
- Last Funding Amount: $47M (Latest Round: February 13, 2024)
- Total Funding Amount: $47M
Antithesis specializes in leveraging AI-driven solutions to provide advanced cybersecurity and data protection services. Their platform focuses on automating threat detection and mitigation, allowing organizations to proactively safeguard sensitive data. With a deep expertise in machine learning and data analytics, Antithesis delivers customized security solutions that adapt to evolving cyber threats. Their technology helps clients enhance their security posture while optimizing risk management in a dynamic, data-driven environment.
12. Rain AI
- Founded: June 2017
- Location: Redwood City, California, USA
- Industry: Artificial Intelligence (AI), Hardware
- Number of Employees: 11–50
- Last Funding Amount: $8.1M (Latest Round: May 26, 2024)
- Total Funding Amount: $40.23M
Rain AI is focused on building AI-powered hardware solutions to optimize performance in data-intensive industries. The company combines cutting-edge machine learning algorithms with innovative hardware design to enhance processing efficiency, automate tasks, and enable real-time decision-making. Their technology is particularly applied in industries like manufacturing, automotive, and cloud computing, helping businesses increase productivity and reduce operational costs while improving scalability and flexibility.
13. Reflexivity
- Founded: February 2019
- Location: New York, New York, USA
- Industry: Artificial Intelligence (AI), Cryptocurrency
- Number of Employees: 11–50
- Last Funding Amount: $30M (Latest Round: October 29, 2024)
- Total Funding Amount: $42.75M
Reflexivity leverages AI to optimize cryptocurrency trading and investment strategies. The company combines advanced machine learning models with real-time market data to create predictive analytics tools for crypto traders and institutional investors. Their platform helps clients enhance decision-making by analyzing market trends, identifying opportunities, and mitigating risks. Reflexivity aims to bring the power of artificial intelligence to the fast-paced world of cryptocurrency, enhancing both speed and precision in trading strategies.
14. PictorLabs
- Founded: June 6, 2019
- Location: Los Angeles, California, USA
- Industry: Artificial Intelligence (AI), Biopharma
- Number of Employees: 11–50
- Last Funding Amount: $30M (Latest Round: September 18, 2024)
- Total Funding Amount: $48.815M
PictorLabs uses advanced AI and machine learning techniques to enhance the drug discovery and development process in the biopharma industry. Their platform leverages vast datasets to predict protein structures, identify drug targets, and optimize clinical trial designs. PictorLabs aims to accelerate the development of new treatments and reduce costs in the biopharma industry by providing more accurate insights and predictions for researchers and pharmaceutical companies.
15. Segmed
- Founded: May 2019
- Location: Palo Alto, California, USA
- Industry: Artificial Intelligence (AI), Cloud Data Services
- Number of Employees: 11–50
- Last Funding Amount: $10.4M (Latest Round: September 3, 2024)
- Total Funding Amount: $20.42M
Segmed specializes in using AI to enhance medical imaging and healthcare data processing. Their cloud-based platform aggregates and analyzes large datasets of medical images and clinical information to support research and clinical decision-making. By leveraging AI and deep learning techniques, Segmed helps healthcare providers improve diagnostic accuracy and accelerates the development of AI-powered healthcare solutions.
16. Bolster AI
- Founded: March 2019
- Location: Santa Clara, California, USA
- Industry: Artificial Intelligence (AI), Cyber Security
- Number of Employees: 51–100
- Last Funding Amount: $14M (Latest Round: May 22, 2024)
- Total Funding Amount: $46M
Bolster AI provides AI-driven cybersecurity solutions that focus on preventing fraud, identity theft, and other online threats. Their platform uses machine learning algorithms to detect and mitigate advanced cyber threats in real-time, providing businesses with proactive defense mechanisms. By automating threat detection and response, Bolster AI enhances security and helps organizations safeguard their digital assets and customer data.
17. Epistemic AI
- Founded: July 2018
- Location: Westport, Connecticut, USA
- Industry: Artificial Intelligence (AI), Bioinformatics
- Number of Employees: 11–50
- Last Funding Amount: $4M (Latest Round: Jan 13, 2025)
- Total Funding Amount: $11.39M
Epistemic AI leverages artificial intelligence to advance the field of bioinformatics, focusing on analyzing and interpreting complex biological data. Their platform uses machine learning techniques to support drug discovery, precision medicine, and genomic research. By combining AI with bioinformatics, Epistemic AI aims to accelerate the understanding of biological processes and provide actionable insights for improving healthcare and medical treatments.
18. Numa
- Founded: 2017
- Location: Oakland, California, USA
- Industry: Artificial Intelligence (AI), Information Technology
- Number of Employees: 11–50
- Last Funding Amount: $32M (Latest Round: Oct 1, 2024)
- Total Funding Amount: $44.1M
Numa utilizes AI-driven solutions to enhance information technology infrastructure, focusing on improving workflows and automating processes. Their platform helps organizations integrate AI into their IT systems, enabling smarter decision-making, enhanced operational efficiency, and better resource management. With significant funding, Numa is poised to further develop its AI capabilities to help businesses leverage technology in more advanced and impactful ways.
19. Ezra AI
- Founded: 2018
- Location: New York, New York, USA
- Industry: Artificial Intelligence (AI), Health Care
- Number of Employees: 11–50
- Last Funding Amount: $21M (Latest Round: Feb 7, 2024)
- Total Funding Amount: $43M
Ezra AI focuses on utilizing artificial intelligence to improve healthcare outcomes, particularly in the early detection of diseases through advanced imaging and diagnostics. Their platform aims to revolutionize medical practices by automating image analysis, offering faster and more accurate diagnoses. With significant backing, Ezra AI is positioning itself to make a major impact in the healthcare industry by helping clinicians and healthcare providers make more informed decisions and providing better patient outcomes.
20. Reveal Technology
- Founded: 2018
- Location: San Francisco, California, USA
- Industry: Artificial Intelligence (AI)
- Number of Employees: 11–50
- Last Funding Amount: $11.2M (Latest Round: Dec 11, 2024)
- Total Funding Amount: $11.95M
Reveal Technology focuses on leveraging AI to transform data analysis and business intelligence. Their platform specializes in automating the extraction of valuable insights from complex datasets, helping organizations make data-driven decisions faster and more accurately. By integrating machine learning and AI-driven algorithms, Reveal Technology offers innovative solutions that enhance operational efficiency across various industries. With the latest funding, the company is poised for significant expansion in the AI-driven analytics space.
Conclusion
Armed with $10M–$50M in funding, this new generation of U.S.-based AI startups is transforming breakthrough research into powerful, practical solutions. From ethical AI and healthcare innovation to advanced automation and data intelligence, they’re not just riding the AI wave—they’re steering it. With bold ideas and strong execution, these companies are defining the future of American AI.
FAQs
Q1. What types of challenges are they solving?
A. They’re addressing complex issues in healthcare diagnostics, financial modeling, logistics optimization, cybersecurity, and responsible AI deployment.
Q2. How are they using their $10M–$50M in funding?
A. To grow their teams, scale products, accelerate R&D, secure partnerships, and bring enterprise-ready AI tools to market.
Q3. Who is investing in these startups?
A. Top-tier VCs, corporate venture arms, AI-focused funds, and tech-savvy angel investors are fueling their growth.